Siderophore Profiling Using Immobilized Metal Affinity Chromatography
Najwa Braich
Universitey of Sydney, Blackburn bld, Camperdown, The University of Sydney, NSW 2006, Australia
Abstract:
Siderophores are low-molecular-weight secondary metabolites produced by microorganisms and plants for Fe(III) acquisition. Examples of pharmacologically important siderophores are desferrioxamine B (DFO B) or ‘Desferal’ used in the treatment of iron overload diseases; and trichostatin A, which is a potent histone deacetylase inhibitor.
The discovery of new siderophores is hindered by low yields from native sources. In this study, a fast and effective means of siderophore profiling was achieved using Ni2+-charged immobilized metal ion affinity chromatography (IMAC). The siderophore profiling technique was used successfully to capture highly pure DFO B and trichostatin A from crude samples of culture supernatants of Streptomyces pilosus and Streptomyces hygroscopicus, respectively.
Salinispora tropica is a marine actinomycete. The genome of S. tropica is predicted by bioinformatics to encode for multiple siderophores, including DFO- and yersiniabactin-like siderophores.1 In this study, it was found that S. tropica produces more then 12 siderophore-like molecules. Of these, 6 have been identified as DFO B, DFO A1, DFO A2, DFO D1, DFO D2 and DFO E. The siderophore profiling technique expedites sideophore mapping from bacterial culture and will accelerate siderophore biodiscovery. The aqueous compatible, green technology has the potential to streamline biodiscovery and pharmaceutics processing.
